ATG9A Monoclonal antibody

ATG9A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D12E11

Cat no : 67096-1-Ig

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67096-1-Ig targets ATG9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG9A fusion protein Ag24278
Full Name ATG9 autophagy related 9 homolog A (S. cerevisiae)
Calculated Molecular Weight 837 aa, 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC065534
Gene Symbol ATG9A
Gene ID (NCBI) 79065
RRIDAB_2882401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)

Protocols

Product Specific Protocols
WB protocol for ATG9A antibody 67096-1-IgDownload protocol
IHC protocol for ATG9A antibody 67096-1-IgDownload protocol
IF protocol for ATG9A antibody 67096-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

NPJ Parkinsons Dis

Regulators of proteostasis are translationally repressed in fibroblasts from patients with sporadic and LRRK2-G2019S Parkinson's disease

Authors - Dani Flinkman
mouseWB

Invest Ophthalmol Vis Sci

HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation

Authors - Jing Zhang
IF

Cell Rep

Apolipoproteins L1 and L3 control mitochondrial membrane dynamics

Authors - Laurence Lecordier
human,mouseWB

Drug Resist Updat

NDRG1 overcomes resistance to immunotherapy of pancreatic ductal adenocarcinoma through inhibiting ATG9A-dependent degradation of MHC-1

Authors - Zhiheng Zhang
humanWB,IF

Nat Commun

Local membrane source gathering by p62 body drives autophagosome formation

Authors - Xuezhao Feng