ATG9A Monoclonal antibody

ATG9A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1D12E11

Cat no : 67096-1-Ig

Synonyms

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

---

Validation Data Gallery

• 

  WB analysis using 67096-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67096-1-Ig (ATG9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 67096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 67096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67096-1-Ig (ATG9A antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67096-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67096-1-Ig (ATG9A antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below

Product Information

67096-1-Ig targets ATG9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATG9A fusion protein Ag24278
• Full Name: ATG9 autophagy related 9 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 837 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC065534
• Gene Symbol: ATG9A
• Gene ID (NCBI): 79065
• RRID: AB_2882401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)

Publications

Species	Application	Title
human	WB	NPJ Parkinsons Dis; Regulators of proteostasis are translationally repressed in fibroblasts from patients with sporadic and LRRK2-G2019S Parkinson's disease; Authors - Dani Flinkman
mouse	WB	Invest Ophthalmol Vis Sci; HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation; Authors - Jing Zhang
	IF	Cell Rep; Apolipoproteins L1 and L3 control mitochondrial membrane dynamics; Authors - Laurence Lecordier
human,mouse	WB	Drug Resist Updat; NDRG1 overcomes resistance to immunotherapy of pancreatic ductal adenocarcinoma through inhibiting ATG9A-dependent degradation of MHC-1; Authors - Zhiheng Zhang
human	WB,IF	Nat Commun; Local membrane source gathering by p62 body drives autophagosome formation; Authors - Xuezhao Feng