Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,Sqstm1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,Sqstm1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 84826-1-RR
rat brain tissue were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 84826-1-RR
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of NIH/3T3 using 84826-1-RR
IP result of anti-P62,SQSTM1 (IP:84826-1-RR, 4ug; Detection:84826-1-RR 1:4000) with NIH/3T3 cells lysate 1920 ug.
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NIH/3T3 cells, HeLa cells, rat brain tissue, HepG2 cells, Jurkat cells, Raji cells, HEK-293T cells, MCF-7 cells
Positive IP detected in
NIH/3T3 cells
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
NIH/3T3 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84826-1-RR targets P62,SQSTM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.
Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,Sqstm1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 84826-1-RR
rat brain tissue were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 84826-1-RR
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 84826-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 84826-1-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62,SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of NIH/3T3 using 84826-1-RR
IP result of anti-P62,SQSTM1 (IP:84826-1-RR, 4ug; Detection:84826-1-RR 1:4000) with NIH/3T3 cells lysate 1920 ug.
IF/ICC Figures
IF Staining of NIH/3T3 using 84826-1-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using P62,SQSTM1 antibody (84826-1-RR, Clone: 241992C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
AFFINITY Figures
Affinity and Kinetic Characterization of 84826-1-RR
Biolayer interferometry (BLl) kinetic assays of 84826-1-RR against Mouse P62,SQSTM1 were performed. The affinity constant is 0.203 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
P62,SQSTM1 Recombinant antibody
Catalog Number
84826-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:250-1:1000
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
