• Featured Product
  • KD/KO Validated

ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 11264-1-AP


APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12

Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, NIH/3T3 cells, HeLa cells, HCT 116 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11264-1-AP targets ATG12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG12 fusion protein Ag1791
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC012266
Gene Symbol ATG12
Gene ID (NCBI) 9140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.


Product Specific Protocols
WB protocol for ATG12 antibody 11264-1-APDownload protocol
IHC protocol for ATG12 antibody 11264-1-APDownload protocol
IF protocol for ATG12 antibody 11264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Cell Dev Biol

Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat

Authors - Huangrong Zhu

Biochem J

Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.

Authors - Chunyang Du

Biology (Basel)

Autophagy Alters Bladder Angiogenesis and Improves Bladder Hyperactivity in the Pathogenesis of Ketamine-Induced Cystitis in a Rat Model.

Authors - Jian-He Lu

Int J Biochem Cell Biol

Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy.

Authors - Chunyang Du

Eur J Pharmacol

Latcripin-7A from Lentinula edodes C91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling.

Authors - Syed Riaz Ud Din


LncRNA DDX11-AS1 Promotes Chemoresistance through LIN28A-Mediated ATG12 mRNA Stabilization in Breast Cancer

Authors - Xinxin Si