CD107a / LAMP1 Polyclonal antibody

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  WB analysis using 21997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21997-1-AP (LAMP1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21997-1-AP (LAMP1 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of human ovary tumor using 21997-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CD107a / LAMP1 antibody (21997-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD107a / LAMP1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 21997-1-AP

Publications(157)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1

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Tested Applications

• Positive WB detected in: HepG2 cells, U937 cells
• Positive IHC detected in: human colon cancer tissue, human gliomas tissue, human liver cancer tissue, human pancreas tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human ovary tumor tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 102 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

21997-1-AP targets CD107a / LAMP1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, monkey, zebrafish, bovine, turtle
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17367

  Product name: Recombinant human CD107a protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 35-377 aa of BC021288

  Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLL

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC021288
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• RRID: AB_2878966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Immunity; The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.; Authors - Meihong Deng
human		Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human		Adv Sci (Weinh); OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model; Authors - Xiaomeng Song
	WB	Arthritis Rheumatol; LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands; Authors - Hiroyuki Nakamura
human		Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
human		Nat Commun; Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer.; Authors - Nindo B Punturi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zakia (Verified Customer) (05-22-2024)

Works great.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: N2A,293T cells, IMG

FH

Marina (Verified Customer) (08-08-2022)

Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human breast cancer cells

CD107a / LAMP1 Antibody Western Blot validation (1:1000 dilution) in Human breast cancer cells (Cat no:21997-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

Mayru (Verified Customer) (04-30-2019)

The antibody works great for detection of LAMP1 protein expression using Western blot analysis.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H4 cells