ATG5 Recombinant antibody

• Featured Product
• KD/KO Validated

ATG5 Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5M5

Cat no : 81803-1-RR

Synonyms

Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE, APG5

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Validation Data Gallery

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  WB analysis of HeLa using 81803-1-RR

  WB result of ATG5 antibody (81803-1-RR; 1:6000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells.

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  WB analysis using 81803-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 81803-1-RR

  IP result of anti-ATG5 (IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1280 ug.

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  IHC staining of human ovary tumor using 81803-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 81803-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 81803-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81803-1-RR against Human ATG5 were performed. The affinity constant is 42.4 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, A549 cells, NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells, PC-12 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81803-1-RR targets ATG5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATG5 fusion protein Ag0214
• Full Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC002699
• Gene Symbol: ATG5
• Gene ID (NCBI): 9474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band.