ULK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ULK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

63

Cat no : 20986-1-AP

Synonyms

Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

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Validation Data Gallery

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  WB analysis of HepG2 using 20986-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 20986-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20986-1-AP (ULK1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20986-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20986-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20986-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (20986-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HepG2 cells, HEK-293T cells, rat skeletal muscle tissue, HeLa cells
• Positive IHC detected in: human skeletal muscle tissue, human liver cancer tissue, human liver tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 58 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

20986-1-AP targets ULK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-51-like kinase 1 (C. elegans)
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 113-140 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• RRID: AB_2878783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Publications

Species	Application	Title
human	IHC	Cancer Cell; Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.; Authors - Hua Su
human	WB	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan ; KO Validated
human	WB	Autophagy; Topology-dependent, bifurcated mitochondrial quality control under starvation.; Authors - Yanshuang Zhou
human	IF	Dev Cell; Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner.; Authors - Cansu Karabiyik
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
mouse	WB	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane