Anticorps Polyclonal de lapin anti-Cyclin E1

• Phare
• Validé par KD/KO

Cyclin E1 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, FC (Intra), CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

290

Avis (2)

N° de cat : 11554-1-AP

Synonymes

CCNE1, G1/S-specific cyclin-E1, G1/S specific cyclin E1, Cyclin E, CCNE

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Galerie de données de validation

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  WB analysis using 11554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11554-1-AP

  Non-treated HeLa and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis of mouse heart using 11554-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11554-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11554-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11554-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11554-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11554-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11554-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 11554-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of MCF-7 using 11554-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, tissu cardiaque de souris, tissu placentaire humain, tissu pulmonaire humain
• Résultats positifs en IHC: tissu testiculaire de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 279 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11554-1-AP cible Cyclin E1 dans les applications de WB, IHC, FC (Intra), CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclin E1 Protéine recombinante Ag2110
• Nom complet: cyclin E1
• Masse moléculaire calculée: 410 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC035498
• Symbole du gène: Cyclin E1
• Identification du gène (NCBI): 898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).

Publications

Species	Application	Title
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
mouse	IHC	Theranostics; Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction; Authors - Meng Gao
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
mouse	WB	Nat Commun; Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses.; Authors - Yuhang Jiang
human	WB	EBioMedicine; Shuttling SLC2A4RG is regulated by 14-3-3θ to modulate cell survival via caspase-3 and caspase-6 in human glioma.; Authors - Dapeng Yun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (08-04-2024)

Antibody detect a weak band around 50kDa, however the signal is weak and non-specific strong signal is present at ~75kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

Cyclin E1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:11554-1-AP)

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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