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P21 Polyclonal antibody
P21 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
780
Cat no : 10355-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, THP-1 cells, LNCaP cells, NCCIT cells, U2OS cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | myotome of a somite at E11.5 tissue |
Positive IF/ICC detected in | MCF-7 cells, myotome of a somite at E11.5 tissue |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:10-1:100 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
10355-1-AP targets P21 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, pig, rabbit, canine, bovine, artemia sinica |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | P21 fusion protein Ag0368 |
Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
Calculated Molecular Weight | 18 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC000275 |
Gene Symbol | p21 |
Gene ID (NCBI) | 1026 |
RRID | AB_2077682 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Protocols
Product Specific Protocols | |
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WB protocol for P21 antibody 10355-1-AP | Download protocol |
IHC protocol for P21 antibody 10355-1-AP | Download protocol |
IF protocol for P21 antibody 10355-1-AP | Download protocol |
IP protocol for P21 antibody 10355-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Gastroenterology Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6. | ||
Sci Transl Med Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer | ||
Nat Commun Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity | ||
Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Mol Cancer Circular RNA hsa_circ_0004872 inhibits gastric cancer progression via the miR-224/Smad4/ADAR1 successive regulatory circuit. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Vikas (Verified Customer) (04-23-2024) | Used p21 antibody at 1:1000 dilution for western blotting worked great. Thank You. Highly recommended.
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FH Hadil (Verified Customer) (05-24-2023) | I prepared this antibody in odyssey blocker at 1:1000 and incubated at 4 C overnight and seemed to get a good signal for p21. There were some non-specific bands properly caused by long incubation periods. So, i would recommend using it at this concentration for 1.5-2 hr at RT.
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FH Malak (Verified Customer) (04-22-2021) | The best incubation period for this antibody is 1.5 hr at room temperature, longer incubations (especially overnight at 4°C) may result in aspecific bands.
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FH Susan (Verified Customer) (12-03-2019) | We had been having issues with our old P21 antibody and this one worked great.
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FH Béatrice (Verified Customer) (09-04-2019) | This is an excellent and specific antibody, without background.When prepared in 3%BSA, it can be reused several times and kept at-20°C
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