Cyclin E2 Polyclonal antibody

Cyclin E2 Polyclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 11935-1-AP


CCNE2, CYCE2, Cyclin E, cyclin E2, G1/S specific cyclin E2

Tested Applications

Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11935-1-AP targets Cyclin E2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin E2 fusion protein Ag2532
Full Name cyclin E2
Calculated Molecular Weight 374 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC020729
Gene Symbol CCNE2
Gene ID (NCBI) 9134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.


Product Specific Protocols
IHC protocol for Cyclin E2 antibody 11935-1-APDownload protocol
IF protocol for Cyclin E2 antibody 11935-1-APDownload protocol
IP protocol for Cyclin E2 antibody 11935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin


PMID: 30132758

Authors - Tonya Kueck

Mol Ther Nucleic Acids

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Authors - Yalei Cui

Mol Ther Nucleic Acids

lncRNA STEAP3-AS1 Modulates Cell Cycle Progression via Affecting CDKN1C Expression through STEAP3 in Colon Cancer.

Authors - Heya Na


Upregulation of E2F8 promotes cell proliferation and tumorigenicity in breast cancer by modulating G1/S phase transition.

Authors - Liping Ye
humanWB, IHC

Cancers (Basel)

A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.

Authors - Chiao-Yun Lin


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Juri (Verified Customer) (12-22-2023)

Worked ok with immunocytochemistry

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 in 200
  • Cell Tissue Type: Cell