CDK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

283

Reviews (1)

Cat no : 10122-1-AP

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

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Validation Data Gallery

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  WB analysis using 10122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10122-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10122-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10122-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 10122-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10122-1-AP

  IP result of anti-CDK2 (IP:10122-1-AP, 4ug; Detection:10122-1-AP 1:10000) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 10122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CDK2 antibody (10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10122-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (10122-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 10122-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (10122-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue, HEK-293 cells, Hela cells, Jurkat cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, human prostate cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 276 publications below
• IHC: See 14 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below

Product Information

10122-1-AP targets CDK2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, sheep, fish, sea cucumbers
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK2 fusion protein Ag0166
• Full Name: cyclin-dependent kinase 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC003065
• Gene Symbol: CDK2
• Gene ID (NCBI): 1017
• RRID: AB_2078556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,CoIP	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo ; KD Validated
human	WB,IHC	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
human	WB	Sci Adv; Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer; Authors - Atish Mohanty
human	WB	Nat Commun; Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.; Authors - Xiaoyu Ma
human	WB	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Silvia (Verified Customer) (10-19-2021)

It works well for WB

• Applications: Western Blot
• Cell Tissue Type: Human fibroblasts

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