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- KD/KO Validated
CDK2 Polyclonal antibody
CDK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10122-1-AP
Validation Data Gallery
|Positive WB detected in
|HepG2 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue, HEK-293 cells, Jurkat cells, K-562 cells, Hela cells
|Positive IP detected in
|Positive IHC detected in
|human liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, mouse testis tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10122-1-AP targets CDK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, sheep, sea cucumbers, fish, zebrafish
|Host / Isotype
|Rabbit / IgG
|CDK2 fusion protein Ag0166
|cyclin-dependent kinase 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols
|WB protocol for CDK2 antibody 10122-1-AP
|IHC protocol for CDK2 antibody 10122-1-AP
|IF protocol for CDK2 antibody 10122-1-AP
|IP protocol for CDK2 antibody 10122-1-AP
|Click here to view our Standard Protocols
Signal Transduct Target Ther
SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer
Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.
J Clin Invest
TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.
Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Silvia (Verified Customer) (10-19-2021)
It works well for WB