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  • KD/KO Validated

P21 Monoclonal antibody

P21 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b


monkey and More (1)







Cat no : 60214-1-Ig


CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60214-1-Ig is P21 Fusion Protein expressed in E. coli.

Tested Reactivity monkey
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P21 fusion protein Ag0308
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC000312
Gene Symbol CDKN1A
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.


Product Specific Protocols
WB protocol for P21 antibody 60214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

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Authors - Rong Wang

Biomed Pharmacother

MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.

Authors - Xiangdong Bai

J Pathol

NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.

Authors - Rong-Zong Liu
  • KD Validated


Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie

Aging (Albany NY)

Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation.

Authors - Rika Imai

J Biol Chem

RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.

Authors - Xin Lin


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ying (Verified Customer) (02-02-2021)

it is very good antibody.

  • Applications: Western Blot

Chunxia (Verified Customer) (02-02-2021)

Tissue culture

  • Applications: Western Blot, Cell culture