RB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RB1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 17218-1-AP

Synonyms

OSRC, p105 Rb, pp110, pRb, Rb, RB1, retinoblastoma 1

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Validation Data Gallery

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  WB analysis of HT-1080 using 17218-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 17218-1-AP (RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 17218-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 17218-1-AP (RB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HT-1080 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below

Product Information

17218-1-AP targets RB1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RB1 fusion protein Ag11035
• Full Name: retinoblastoma 1
• Calculated Molecular Weight: 928 aa, 106 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC039060
• Gene Symbol: RB1
• Gene ID (NCBI): 5925
• RRID: AB_2177206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.

Publications

Species	Application	Title
human	WB	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu
	WB	J Hazard Mater; Neurotoxicity of aluminum oxide nanoparticles and their mechanistic role in dopaminergic neuron injury involving p53-related pathways.; Authors - Huanliang Liu
human	WB	Aging Cell; Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression.; Authors - Yao Dang
zebrafish	WB	Development; Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1.; Authors - Zili Zhang ; KO Validated
human	WB	Oncotarget; High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.; Authors - Delong Meng
human	WB	Oncotarget; Drp1-mediated mitochondrial fission promotes cell proliferation through crosstalk of p53 and NF-κB pathways in hepatocellular carcinoma.; Authors - Lei Zhan

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