FBXW7 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBXW7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 55290-1-AP

Synonyms

AGO, Archipelago homolog, CDC4, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, hAgo, hCdc4, SEL 10, SEL10

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Validation Data Gallery

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  WB analysis of HEK-293 using 55290-1-AP

  WB result of FBXW7 antibody (55290-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXW7 transfected HEK-293 cells.

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  WB analysis using 55290-1-AP

  HepG2 cell lysates were subjected to SDS PAGE followed by western blot with 55290-1-AP (FBXW7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55290-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55290-1-AP (FBXW7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55290-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 55290-1-AP (FBXW7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55290-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55290-1-AP (FBXW7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 55290-1-AP

  IP result of anti-FBXW7 (IP:55290-1-AP, 4ug; Detection:55290-1-AP 1:500) with HepG2 cells lysate 960 ug.

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  IHC staining of human skin cancer using 55290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55290-1-AP (FBXW7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55290-1-AP (FBXW7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55290-1-AP (FBXW7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 55290-1-AP (FBXW7 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human skin cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IP: See 2 publications below

Product Information

55290-1-AP targets FBXW7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: F-box and WD repeat domain containing 7
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 100-110 kDa, 66-75 kDa
• GenBank Accession Number: NM_018315
• Gene Symbol: FBXW7
• Gene ID (NCBI): 55294
• RRID: AB_2881300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.

Publications

Species	Application	Title
human	WB	Cell; Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication; Authors - Jingkun Zeng ; KD Validated
human	WB	iScience; Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal; Authors - Da-Wei Yeh
human	WB	Oncotarget; PMID: 28404959; Authors - Min Zheng
mouse	WB	Endocrinology; Rictor regulates spermatogenesis by controlling Sertoli cell cytoskeletal organization and cell polarity in the mouse testis.; Authors - Heling Dong
human	WB	Int J Biochem Cell Biol; The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc.; Authors - Zhijun Xu
human	WB	Biochim Biophys Acta Gen Subj; FBXO8 is a novel prognostic biomarker in different molecular subtypes of breast cancer and suppresses breast cancer progression by targeting c-MYC; Authors - Abdul Jamil Khan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (09-17-2020)

FBX W7 is an excellent antibody for WB and IHC. Giving a very clear bands and sharp staining in tissue sections.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer