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P27; KIP1 Polyclonal antibody
P27; KIP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 25614-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, NIH/3T3 cells, HepG2 cells, MCF-7 cells, Jurkat cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse testis tissue|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
25614-1-AP targets P27; KIP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, sheep, canine, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P27; KIP1 fusion protein Ag22582|
|Full Name||cyclin-dependent kinase inhibitor 1B (p27, Kip1)|
|Calculated molecular weight||198 aa, 22 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC001971|
|Gene ID (NCBI)||1027|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.
|Product Specific Protocols|
|WB protocol for P27; KIP1 antibody 25614-1-AP||Download protocol|
|IHC protocol for P27; KIP1 antibody 25614-1-AP||Download protocol|
|IF protocol for P27; KIP1 antibody 25614-1-AP||Download protocol|
|IP protocol for P27; KIP1 antibody 25614-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Malak (Verified Customer) (04-22-2021)
The optimal incubation period is 1.5 hr at room temperature. Avoid incubating the antibody overnight as it may result in aspecific signals.
Susmita (Verified Customer) (09-17-2020)
Antibody is working very good for WB
Joshua (Verified Customer) (07-27-2019)
Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Decent staining with some background in rabbit cells. Nuclear localization as expected.