Cyclin D3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin D3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 26755-1-AP

Synonyms

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

---

Validation Data Gallery

• 

  WB analysis of C6 using 26755-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26755-1-AP

  WB result of Cyclin D3 antibody (26755-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.

• 

  WB analysis of HeLa using 26755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26755-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26755-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 26755-1-AP

  IP result of anti-Cyclin D3 (IP:26755-1-AP, 4ug; Detection:26755-1-AP 1:1000) with K-562 cells lysate 2800ug.

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 26755-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: C6 cells, K-562 cells, Jurkat cells, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human pancreas tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 31 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Product Information

26755-1-AP targets Cyclin D3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin D3 fusion protein Ag25151
• Full Name: cyclin D3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC011616
• Gene Symbol: Cyclin D3
• Gene ID (NCBI): 896
• RRID: AB_2756524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Publications

Species	Application	Title
human	WB,IP	Acta Pharmacol Sin; Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation; Authors - Yu-Jia Xu ; KD Validated
human	WB	J Proteome Res; Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells; Authors - Alireza Delfarah
human	WB	Transl Lung Cancer Res; Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1.; Authors - Zhiwei Zhang
human	IHC	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
mouse	WB	J Cell Mol Med; Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1.; Authors - Dan Zhang
human	WB	Mol Ther Oncolytics; S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression.; Authors - Weijing He