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  • KD/KO Validated

Cyclin D3 Polyclonal antibody

Cyclin D3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (1)





Cat no : 26755-1-AP


CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

Tested Applications

Positive WB detected inC6 cells, K-562 cells, Jurkat cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman pancreas tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26755-1-AP targets Cyclin D3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin D3 fusion protein Ag25151
Full Name cyclin D3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC011616
Gene Symbol CCND3
Gene ID (NCBI) 896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.


Product Specific Protocols
WB protocol for Cyclin D3 antibody 26755-1-APDownload protocol
IHC protocol for Cyclin D3 antibody 26755-1-APDownload protocol
IF protocol for Cyclin D3 antibody 26755-1-APDownload protocol
IP protocol for Cyclin D3 antibody 26755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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