Cyclin D3 Polyclonal antibody

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• Cyclin D3 Polyclonal antibody (26755-1-AP)

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• KD/KO Validated

Cyclin D3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 26755-1-AP Copied!

Publications (48)

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Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

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  WB analysis of C6 using 26755-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 26755-1-AP

  WB result of Cyclin D3 antibody (26755-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 26755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 26755-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 26755-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 26755-1-AP

  IP result of anti-Cyclin D3 (IP:26755-1-AP, 4ug; Detection:26755-1-AP 1:1000) with K-562 cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 26755-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C6 cells, K-562 cells, Jurkat cells, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human pancreas tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26755-1-AP targets Cyclin D3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25151

  Product name: Recombinant human Cyclin D3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 250-292 aa of BC011616

  Sequence: ALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL

• Full Name: cyclin D3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC011616
• Gene Symbol: Cyclin D3
• Gene ID (NCBI): 896
• ENSEMBL Gene ID: ENSG00000112576
• RRID: AB_2756524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30281
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Publications

What published studies show

Anti-Cyclin D3 antibody (Cat# 26755-1-AP) has 48 citations published across 40+ journals, including Molecular Cell, Science Advances, Cell Death & Differentiation, Journal of Experimental & Clinical Cancer Research, Small, and BMC Cancer. Research fields span oncology (lung, breast, gastric, liver, colorectal, and other cancers), cell cycle regulation, DNA mismatch repair, ferroptosis/cuproptosis, diabetic wound healing, stem cell biology, and cardiovascular disease.

Automatically generated from the 48 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Bioact Mater; Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis; Authors - Wenqian Zhang
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
human	WB	Nucleic Acids Res; Small nucleolar RNAs promote the restoration of muscle differentiation defects in cells from myotonic dystrophy type 1; Authors - Baptiste Bogard
human	WB	J Exp Clin Cancer Res; HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.; Authors - Kai Guo
human	WB	Sci Adv; Mechanism of D-type cyclin recognition by the AMBRA1 E3 ligase receptor; Authors - Yang Wang
human	WB	Cell Death Differ; The LINC01315-encoded small protein YAPer-ORF competes with PRP4k to hijack YAP signaling to aberrantly promote cell growth; Authors - Zhu Xie