Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L)
Multi-rAb Goat Anti-rabbit recombinant secondary antibody is a mixture of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on rabbit IgG heavy and light chain.
Applications
IF, FC
Conjugate
CoraLite® Plus 488
Host
Goat
Reactivity
Rabbit
Cat no : RGAR002
Validation Data Gallery
Product Information
RGAR002 targets Rabbit IgG (H+L) in FC and IF. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig and bovine IgGs.
Concentration | 0.5 mg/mL |
Applications | IF, FC |
Host Species | Goat |
Species Reactivity | Rabbit |
Physical State | Liquid |
Suggested Dilution Range | 1:200-1:1000 for IF and FC |
Purity | The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads. |
Storage Buffer | PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4. |
Storage Instructions | Store at -20°C. Stable for one year after shipment. |
Fluorophore | CoraLite® Plus 488, Amax=493 nm, Emax=522 nm |
Clonality | Multiclonal recombinant |
RRID | AB_3073506 |
Note | This product is for research use only,not for diagnostic or therapeutic use. |
Background
Publications
Title |
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Drug Resist Updat Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestration |
J Neurophysiol The effect of repetitive magnetic stimulation on neuronal apoptosis and PI3K/Akt protein expression in rats with incomplete spinal cord injury |
Mol Neurobiol Oxidative Stress-mediated Loss of Hippocampal Parvalbumin Interneurons Contributes to Memory Precision Decline After Acute Sleep Deprivation |
Clin Transl Oncol Development of B7-H3 targeted CAR-T cells for renal cell carcinoma therapy: in vitro and in vivo efficacy |
Toxicol Appl Pharmacol PM10 dysregulates epithelial barrier function in human corneal epithelial cells that is restored by antioxidant SKQ1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ramakrushna (Verified Customer) (08-07-2024) | The secondary antibody (RGAR002) was used at a concentration of 1:500 against Rabbit anti-Spc72 in yeast cells fixed for 30min using PFA for Immunofluorescence. In parallel, I also used our routinely used Invitrogen Donkey anti-Rabbit Alexa488 Catalog # A32790 at 1:500. The signal for the Proteintech antibody sample was significantly weaker than the Invitrogen, however, I think it might have to do with the better specificity of the Proteintech antibody because the signal is less dispersed and confined to a point as it should be. I have attached some images: Sample1- Invitrogen secondary used, Sample 2-Proteintech secondary used Goat Anti-Rabbit 488; yeast cells imaged with a Nikon microscope at 60x. Image should be accessible using ImageJ/Fiji.
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FH Wess (Verified Customer) (02-26-2024) | Worked as expected! Clean backgrounds
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