Anticorps Polyclonal de lapin anti-Cytokeratin 8

• Phare
• Validé par KD/KO

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10384-1-AP

Synonymes

KRT8, Cytokeratin-8, Cytokeratin,Keratin, CK-8, CK8

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Galerie de données de validation

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  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10384-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10384-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10384-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10384-1-AP

  IP result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human breast cancer using 10384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules COLO 320, cellules HaCaT, cellules MCF-7, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain, tissu de côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

10384-1-AP cible Cytokeratin 8 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, chien, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 8 Protéine recombinante Ag0488
• Nom complet: keratin 8
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC000654
• Symbole du gène: Cytokeratin 8
• Identification du gène (NCBI): 3856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Publications

Species	Application	Title
human	WB,IF,IP	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network; Authors - Archan Chakraborty ; KD Validated
mouse	WB,IF	Front Cell Dev Biol; ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.; Authors - Shuai Yu
human	WB,IF	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.; Authors - Archan Chakraborty ; KD Validated
human, goat, dog, pig	IHC	Anat Rec (Hoboken); Comparative study of the external auditory canal in humans and large mammals.; Authors - Jun Wang
human	WB,IF	Oncotarget; Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.; Authors - Sike Hu
mouse	IHC	Oncoscience; The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.; Authors - Dhanushka Hewa Bostanthirige

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