Cytokeratin 18 Polyclonal antibody

Cytokeratin 18 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (7)





Cat no : 10830-1-AP


CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

Tested Applications

Positive WB detected inHeLa cells, A431 cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human liver cancer tissue, human kidney tissue, human pancreas cancer tissue, human prostate cancer tissue, human ovary tumor tissue, human stomach cancer tissue, human endometrial cancer tissue, human renal cell carcinoma tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells, mouse olfactory epithelium tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10830-1-AP targets Cytokeratin 18 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat, sheep, monkey, pig, duck, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 18 fusion protein Ag1260
Full Name keratin 18
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000180
Gene Symbol Cytokeratin 18
Gene ID (NCBI) 3875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.


Product Specific Protocols
WB protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IHC protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IF protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
IP protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
FC protocol for Cytokeratin 18 antibody 10830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Parmveer (Verified Customer) (12-02-2019)

Works well for both IHC and IF, but does come with a bit of background

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate
Cytokeratin 18 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1000 dilution) in Prostate (Cat no:10830-1-AP)