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Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10143-1-AP

Synonyms

Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ



Tested Applications

Positive WB detected inA431 cells, rat skin tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human skin tissue, human skin cancer tissue, human oesophagus cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHaCaT cells, HeLa cells, HepG2 cells, mouse olfactory epithelium tissue
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10143-1-AP targets Cytokeratin 14 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag0188
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC002690
Gene symbol KRT14
Gene ID (NCBI) 3861
RRIDAB_2134831
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
IHC protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
IF protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
IP protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
mouseIF

Nat Commun

Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.

Authors - Yue Hong
humanIF

Nat Commun

Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.

Authors - Vlasta Lungova
mouseIF

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
humanWB

Oncogene

EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.

Authors - C-F Yang
mouseIF

Aging Cell

Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.

Authors - Wen-Li Hsu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parmveer (Verified Customer) (10-20-2019)

Worked well to detect basal cells around the prostate gland of formalin fixed tissue

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Prostate
Cytokeratin 14 Antibody Immunofluorescence, validation (1:400 dilution) in Prostate (Cat no:10143-1-AP)
FH

Joshua (Verified Customer) (05-10-2019)

Human Keratinocytes fixed in 4% paraformaldehyde and stained for KRT14. Goat anti-rabbit 488. Bright, specific staining observed.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Cytokeratin 14 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:10143-1-AP)