Cytokeratin 14 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

78

Reviews (3)

Cat no : 10143-1-AP

Synonyms

KRT14, keratin 14, Cytokeratin-14, Cytokeratin,Keratin, CK-14

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Validation Data Gallery

• 

  WB analysis of A431 using 10143-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 10143-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10143-1-AP

  IP result of anti-Cytokeratin 14 (IP:10143-1-AP, 3ug; Detection:10143-1-AP 1:1000) with A431 cells lysate 1200ug.

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human skin using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10143-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10143-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HaCaT using 10143-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10143-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

  IF result of CK14 (10143-1-AP, 1:400) with 1% PLP fixed basal quiescent stem cells of the adult mouse olfactory epithelium. (Green: CK14; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.

• 

  FC experiment of A431 using 10143-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 14 (10143-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, rat skin tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human skin tissue, human skin cancer tissue, human oesophagus cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HaCaT cells, HeLa cells, HepG2 cells, mouse olfactory epithelium tissue
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 23 publications below
• IF: See 49 publications below

Product Information

10143-1-AP targets Cytokeratin 14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 14 fusion protein Ag0188
• Full Name: keratin 14
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC002690
• Gene Symbol: Cytokeratin 14
• Gene ID (NCBI): 3861
• RRID: AB_2134831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Publications

Species	Application	Title
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Nat Commun; Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.; Authors - Yue Hong
human	IF	Nat Commun; Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.; Authors - Vlasta Lungova
mouse	IF	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
human	WB	Oncogene; EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.; Authors - C-F Yang
mouse	IF	Aging Cell; Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.; Authors - Wen-Li Hsu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rachel (Verified Customer) (03-19-2024)

Excellent antibody, got the tester but will be purchasing more for future work.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: MCF10A

Cytokeratin 14 Antibody Western Blot,Immunofluorescence validation (1:200 dilution) in MCF10A (Cat no:10143-1-AP)

FH

Parmveer (Verified Customer) (10-20-2019)

Worked well to detect basal cells around the prostate gland of formalin fixed tissue

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Prostate

Cytokeratin 14 Antibody Immunofluorescence, validation (1:400 dilution) in Prostate (Cat no:10143-1-AP)

FH

Joshua (Verified Customer) (05-10-2019)

Human Keratinocytes fixed in 4% paraformaldehyde and stained for KRT14. Goat anti-rabbit 488. Bright, specific staining observed.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Cytokeratin 14 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:10143-1-AP)

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