Cytokeratin 19 Monoclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 19 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

3G1E4

Cat no : 60187-1-Ig

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Validation Data Gallery

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  WB analysis of MCF-7 using 60187-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 60187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 60187-1-Ig

  WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells.

• 

  WB analysis of HaCaT using 60187-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60187-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 60187-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HaCaT cells, PC-3 cells, HeLa cells, T-47D cells, pig colon tissue, rat colon tissue, mouse colon tissue
• Positive IHC detected in: human colon tissue, human thyroid cancer tissue, human stomach cancer tissue, human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:1000-1:51200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Product Information

60187-1-Ig targets Cytokeratin 19 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• RRID: AB_10859834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
human	WB	Stem Cells; Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells.; Authors - Qiu-Zhong Pan
human	IF,IHC	Stem Cell Res Ther; DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis; Authors - S Y Wang
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	IF	Biomed Pharmacother; Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.; Authors - Xufeng Tao
mouse	IF	Biochim Biophys Acta Mol Basis Dis; TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis; Authors - Yuecheng Guo