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- KD/KO Validated
uPA/Urokinase Polyclonal antibody
uPA/Urokinase Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 17968-1-AP
Validation Data Gallery
|Positive WB detected in
|DU 145 cells, human brain tissue, A375 cells, Hela cells, LNCaP cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human lung tissue, human ovary tissue, human prostate cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17968-1-AP targets uPA/Urokinase in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, bovine
|Host / Isotype
|Rabbit / IgG
|uPA/Urokinase fusion protein Ag11813
|plasminogen activator, urokinase
|Calculated molecular weight
|431 aa, 49 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).
|Product Specific Protocols
|WB protocol for uPA/Urokinase antibody 17968-1-AP
|IHC protocol for uPA/Urokinase antibody 17968-1-AP
|IF protocol for uPA/Urokinase antibody 17968-1-AP
|IP protocol for uPA/Urokinase antibody 17968-1-AP
|Click here to view our Standard Protocols
p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.
Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-mediated Cytokine Release From Myeloid Cells.
J Exp Med
PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.
Aging (Albany NY)
Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.
PDGFD switches on stem cell endothelial commitment.
Aging (Albany NY)
NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.