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uPA/Urokinase Polyclonal antibody

uPA/Urokinase Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 17968-1-AP


ATF, PLAU, U plasminogen activator, uPA, uPA/Urokinase, URK

Tested Applications

Positive WB detected inDU 145 cells, human brain tissue, A375 cells, Hela cells, LNCaP cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung tissue, human ovary tissue, human prostate cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17968-1-AP targets uPA/Urokinase in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen uPA/Urokinase fusion protein Ag11813
Full Name plasminogen activator, urokinase
Calculated Molecular Weight 431 aa, 49 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC013575
Gene Symbol Urokinase
Gene ID (NCBI) 5328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).


Product Specific Protocols
WB protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
IHC protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
IF protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
IP protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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