Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

140

Reviews (1)

Cat no : 10712-1-AP

Synonyms

CK19, CK 19, KRT19, K19, Cytokeratin-19

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Validation Data Gallery

• 

  WB analysis using 10712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10712-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10712-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10712-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10712-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Retinal organoids using 10712-1-AP

  Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

• 

  IF Staining of mouse pancreas using 10712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 10712-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HaCaT using 10712-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10712-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Cytokeratin 19 Polyclonal antibody (10712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, mouse placenta tissue, MCF-7 cells, A549 cells, mouse brain tissue, HT-29 cells
• Positive IHC detected in: human colon tissue, human breast cancer tissue, human lung cancer tissue, human oesophagus cancer tissue, human pancreas tissue, human skin tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse colon tissue, mouse pancreas tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: Retinal organoids, mouse pancreas tissue
• Positive IF/ICC detected in: HaCaT cells, HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:30000
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000
• Immunofluorescence (IF)-P: IF-P : 1:10-1:100
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 27 publications below
• IHC: See 66 publications below
• IF: See 70 publications below
• FC: See 1 publications below

Product Information

10712-1-AP targets Cytokeratin 19 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, goat, dog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag1085
• Full Name: keratin 19
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC007628
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• RRID: AB_2133325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Publications

Species	Application	Title
mouse	IF	ACS Nano; Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition; Authors - Yanan Wang
mouse	IHC,IF	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
human	IHC	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human		Adv Sci (Weinh); PMID: 31728273; Authors - Qingming Tang
human		Nat Commun; Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.; Authors - Xuexiang Han
human	IHC	J Clin Invest; CCN1 induces hepatic ductular reaction through integrin αvβ5-mediated activation of NF-κB.; Authors - Ki-Hyun Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: ROs

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