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  • KD/KO Validated

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, Rat and More (4)





Cat no : 11257-1-AP


ERK, ERK 1, ERK 1/2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, HEK-293 cells, PC-12 cells, C6 cells, mouse brain tissue, mouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells, MCF-7 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, Rat samples.

Tested Reactivity human, mouse, Rat
Cited Reactivityhuman, mouse, rat, sheep, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERK1/2 fusion protein Ag1759
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 43 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC013992
Gene symbol MAPK3
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.


Product Specific Protocols
WB protocol for ERK1/2 antibody 11257-1-APDownload protocol
IHC protocol for ERK1/2 antibody 11257-1-APDownload protocol
IF protocol for ERK1/2 antibody 11257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Precise and efficient silencing of mutant KrasG12D by CRISPR-CasRx controls pancreatic cancer progression.

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


XB (Verified Customer) (10-29-2020)

A reliable ab to detect total ERK1/2 by Western blotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain
ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)