Validation Data Gallery
|Positive WB detected in||NIH/3T3 cells, HeLa cells, HEK-293 cells, PC-12 cells, C6 cells, mouse brain tissue, mouse lung tissue|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A375 cells, MCF-7 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11257-1-AP targets ERK1/2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, Rat samples.
|Tested Reactivity||human, mouse, Rat|
|Cited Reactivity||human, mouse, rat, sheep, pig, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ERK1/2 fusion protein Ag1759|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||38-44 kDa|
|GenBank accession number||BC013992|
|Gene ID (NCBI)||5595|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
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Persistent Degradation of HER2 Protein by Hybrid nanoPROTAC for Programmed Cell Death
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
XB (Verified Customer) (10-29-2020)
A reliable ab to detect total ERK1/2 by Western blotting.