Anticorps Polyclonal de lapin anti-HO-1/HMOX1

• Phare
• Validé par KD/KO

HO-1/HMOX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

931

Avis (4)

N° de cat : 10701-1-AP

Synonymes

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

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Galerie de données de validation

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  WB analysis of HeLa using 10701-1-AP

  WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells.

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  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HMOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10701-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10701-1-AP

  IP result of anti-HO-1/HMOX1 (IP:10701-1-AP, 4ug; Detection:10701-1-AP 1:20000) with HeLa cells lysate 1360 ug.

• 

  IHC staining of mouse spleen using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 10701-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 10701-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10701-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HeLa, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 18 publications below
• WB: See 886 publications below
• IHC: See 77 publications below
• IF: See 65 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

10701-1-AP cible HO-1/HMOX1 dans les applications de WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HO-1/HMOX1 Protéine recombinante Ag1190
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 28-33 kDa
• Numéro d’acquisition GenBank: BC001491
• Symbole du gène: HO-1
• Identification du gène (NCBI): 3162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
human	WB	Cell Res; MFSD7c functions as a transporter of choline at the blood-brain barrier; Authors - Xuan Thi Anh Nguyen
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
	WB	Cell Metab; Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals; Authors - Timothy C Kenny
mouse	WB	Cell Metab; Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.; Authors - Xiphias Ge Zhu
mouse	IHC	ACS Nano; Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway; Authors - Xirui Xin
human	IHC	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Works perfectly. Band a 28-33 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Heart Tissue

HO-1/HMOX1 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:10701-1-AP)

FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it for IF staining of paraffin-embedded spleen tissue. HMOX-1 positive cells are in Green and CD45 in red.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Spleen

HO-1/HMOX1 Antibody Immunofluorescence validation (1/200 dilution) in Spleen (Cat no:10701-1-AP)

FH

Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/3000

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

HO-1/HMOX1 Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:10701-1-AP)

FH

JIM (Verified Customer) (03-26-2019)

This antibody offers a good detection of HO-1 expression in LPS stimulated macrophages. The HO-1 expression can be observed in the cells after 24h stimulation of LPS.

• Applications: Western Blot,

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