BACH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BACH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 14018-1-AP

Synonyms

BACH 1, BACH1, BTB and CNC homolog 1, HA2303

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 14018-1-AP

  WB result of BACH1 antibody (14018-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells.

• 

  WB analysis using 14018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14018-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14018-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14018-1-AP (BACH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 14018-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 14018-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 14018-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14018-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14018-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14018-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14018-1-AP (BACH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, Raji cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human cervical cancer tissue, human breast hyperplasia tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 33 publications below
• IHC: See 12 publications below
• IF: See 12 publications below
• FC: See 1 publications below
• ChIP: See 4 publications below

Product Information

14018-1-AP targets BACH1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BACH1 fusion protein Ag5128
• Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC063307
• Gene Symbol: BACH1
• Gene ID (NCBI): 571
• RRID: AB_2274498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle 

regulation in homeostasis and in cancer in disease.

What is the molecular weight of BACH1?

Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor 

family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs 

anomalously at 100-110 kDa in SDS-PAGE.

What is the cellular localization of BACH1?

As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a 

heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the 

Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by 

heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to 

microtubules (PMID: 15809329).

What is the function of BACH1?

BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme 

oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved 

in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor 

(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a 

role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6 

(CDK6) and BCL2-like 11 (BCL2L11).

What is the role of BACH1 in disease?

BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function 

of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis, 

influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787

892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).

Publications

Species	Application	Title
human	WB,IHC,IF	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human	WB	Autophagy; MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis; Authors - Yuji Shiozaki
human	WB,IF,IHC	Cancer Lett; PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer.; Authors - Tian-Yi Jiang
human	WB	iScience; Regulation of DNA damage response by trimeric G-proteins; Authors - Amer Ali Abd El-Hafeez
mouse	IHC	Front Cell Dev Biol; Single-Cell Analysis Identify Transcription Factor BACH1 as a Master Regulator Gene in Vascular Cells During Aging.; Authors - Fei Ge
human	WB	Virulence; Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation.; Authors - Lei You