ISYNA1 Polyclonal antibody

ISYNA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14142-1-AP

Synonyms

Inositol-3-phosphate synthase 1, INOS, INO1, hIPS, hINO1

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Validation Data Gallery

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  WB analysis of mouse testis using 14142-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14142-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14142-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14142-1-AP

  Immunofluorescent analysis of HepG2 cells, using ISYNA1 antibody 14142-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse testis tissue, HepG2 cells, rat testis tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

14142-1-AP targets ISYNA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ISYNA1 fusion protein Ag5272
• Full Name: inositol-3-phosphate synthase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC066902
• Gene Symbol: ISYNA1
• Gene ID (NCBI): 51477
• RRID: AB_2233801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).

Publications

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.; Authors - Jian-Yi Dong
mouse	WB	Front Pharmacol; Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional Experiments; Authors - Jianyi Dong
rat	WB, IF	Alcohol Clin Exp Res; Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma.; Authors - Keke Yu
mouse	WB	Mol Reprod Dev; Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice.; Authors - Cao Senyang S
	WB	Biomed Pharmacother; Sulforaphane decreases oxidative stress and inhibits NLRP3 inflammasome activation in a mouse model of ulcerative colitis; Authors - Zi-Juan Zhou