VEGFA Polyclonal antibody

• Featured Product
• KD/KO Validated

VEGFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Publications

777

Reviews (3)

Cat no : 19003-1-AP

Synonyms

VEGF, Vascular permeability factor, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor

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Validation Data Gallery

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  WB analysis using 19003-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19003-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19003-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19003-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 19003-1-AP

  IP result of anti-VEGFA (IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19003-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19003-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 19003-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 19003-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, C6 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 79 publications below
• IHC: See 240 publications below
• IF: See 91 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

19003-1-AP targets VEGFA in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFA fusion protein Ag13500
• Full Name: vascular endothelial growth factor A
• Calculated Molecular Weight: 412 aa, 46 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC065522
• Gene Symbol: VEGFA
• Gene ID (NCBI): 7422
• RRID: AB_2212657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Publications

Species	Application	Title
mouse	IHC	Nat Nanotechnol; Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.; Authors - Xuefei Lu ; KD Validated
human		Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
mouse,human		ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
mouse	IHC	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
mouse		Light Sci Appl; Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model.; Authors - Lechan Tao
human	IP	J Clin Invest; Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema.; Authors - Akrit Sodhi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (07-24-2024)

WB result of VEGFA antibody (used at 1:5000) incubated at room temperature for 1.5 hours. Two stronger bands between 35-50 kDa were observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: A549

VEGFA Antibody Western Blot validation (1:5000 dilution) in A549 (Cat no:19003-1-AP)

FH

Alexandru (Verified Customer) (11-21-2023)

Great antibody with clear and specific signal, would buy again!

• Applications: Western Blot
• Cell Tissue Type: Mouse Ventricle

FH

Fiona (Verified Customer) (07-16-2019)

Good quality antibody and good value for money. Would purchase again.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

VEGFA Antibody Western Blot, validation (1:500 dilution) in ARPE-19 (Cat no:19003-1-AP)

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