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  • KD/KO Validated

NRF2, NFE2L2 Polyclonal antibody

NRF2, NFE2L2 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (9)





Cat no : 16396-1-AP


HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2

Tested Applications

Positive WB detected inHepG2 cells, DMSO treated HeLa cells, A549 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human breast cancer tissue, human kidney tissue, human pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMG132 treated HepG2 cells, HepG2 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16396-1-AP targets NRF2, NFE2L2 in WB, IP, IF, FC, IHC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, goat, chicken, yellow catfish, Seiurus aurocapilla, ducks, monkey, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated Molecular Weight 605 aa, 68 kDa
Observed Molecular Weight 110 kDa, 68 kDa
GenBank Accession NumberBC011558
Gene Symbol NFE2L2
Gene ID (NCBI) 4780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).


Product Specific Protocols
WB protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IHC protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IF protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
IP protocol for NRF2, NFE2L2 antibody 16396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guillermo (Verified Customer) (09-03-2022)

Very good product, great spots.

  • Applications: Western Blot

balawant (Verified Customer) (08-06-2022)

for westerblotting I have used this Antibody. this antibody is excellent and I will recommend if you are working with this protein

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: colon tissue and colon cancer cells

Georgina (Verified Customer) (07-04-2022)

This is a good antibody - it gives some background signal but the band of interest is clear. Sometimes need to use a higher concentration than recommended for cells with lower expression of NRF2.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 500 or 1 in 1000
  • Cell Tissue Type: Human HepG2 cells

Iram (Verified Customer) (03-11-2022)

Gave good bands around 100 kda in colon cancer cell lysates

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colon cancer cells

Giovanni (Verified Customer) (01-11-2022)

the antiboby worked very well at dilution 1:1000 in WB using Htr8/SVneo cell line lysates. It gave a specific band at around 100 kDa with very low background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Htr8/SVneo
NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Htr8/SVneo (Cat no:16396-1-AP)

Xiaoping (Verified Customer) (10-14-2021)

Gave good bands around 100 kda in renal cell lysates

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: Kidney

Razia Sultana (Verified Customer) (08-20-2020)

The antibody gives good band at around 100KD(100 KD in HK2 cells and 90KD in kidney tissue lysate) along with some non specific bands at around 40-50KD. The trial size gave a band at 130KD which is unusual and lot of non specific bands,but the full size antibody is good. We are satisfied with the purchase

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 in 5% milk
  • Cell Tissue Type: Rat and mouse kidney tissue lysate and HK2 cell lysate

JIM (Verified Customer) (03-26-2019)

Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.

  • Applications: Western Blot,