NRF2, NFE2L2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NRF2, NFE2L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

1733

Reviews (10)

Cat no : 16396-1-AP

Synonyms

NRF2, NFE2L2, HEBP1, Nrf 2, NFE2-related factor 2

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Validation Data Gallery

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  WB analysis using 16396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16396-1-AP

  DMSO treated HeLa cells were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16396-1-AP

  IP result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 4ug; Detection:16396-1-AP 1:6000) with HeLa cells lysate 1520 ug.

• 

  IHC staining of human pancreas cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16396-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16396-1-AP (NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16396-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16396-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using NRF2, NFE2L2 antibody (16396-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, DMSO treated HeLa cells, A549 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human renal cell carcinoma tissue, human breast cancer tissue, human kidney tissue, human pancreas cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MG132 treated HepG2 cells, HepG2 cells

This antibody is only recommended for WB detection of human samples

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 147 publications below
• WB: See 1590 publications below
• IHC: See 230 publications below
• IF: See 412 publications below
• IP: See 31 publications below
• FC: See 1 publications below
• CoIP: See 22 publications below
• ChIP: See 19 publications below

Product Information

16396-1-AP targets NRF2, NFE2L2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rabbit, monkey, chicken, goat, yellow catfish, seiurus aurocapilla, ducks
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRF2, NFE2L2 fusion protein Ag9489
• Full Name: nuclear factor (erythroid-derived 2)-like 2
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 110 kDa, 68 kDa
• GenBank Accession Number: BC011558
• Gene Symbol: NRF2
• Gene ID (NCBI): 4780
• RRID: AB_2782956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

Species	Application	Title
human,mouse	WB,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB, IP	Cancer Cell; iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.; Authors - Wenjie Ge ; KD Validated
human		Cell Metab; Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.; Authors - Jiajun Zhu
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (08-15-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

NRF2, NFE2L2 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:16396-1-AP)

FH

SD (Verified Customer) (07-05-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

NRF2, NFE2L2 Antibody Western Blot validation (1000 dilution) in A549 (Cat no:16396-1-AP)

FH

Guillermo (Verified Customer) (09-03-2022)

Very good product, great spots.

• Applications: Western Blot

FH

balawant (Verified Customer) (08-06-2022)

for westerblotting I have used this Antibody. this antibody is excellent and I will recommend if you are working with this protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: colon tissue and colon cancer cells

FH

Georgina (Verified Customer) (07-04-2022)

This is a good antibody - it gives some background signal but the band of interest is clear. Sometimes need to use a higher concentration than recommended for cells with lower expression of NRF2.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 500 or 1 in 1000
• Cell Tissue Type: Human HepG2 cells

FH

Iram (Verified Customer) (03-11-2022)

Gave good bands around 100 kda in colon cancer cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Colon cancer cells

FH

Giovanni (Verified Customer) (01-11-2022)

the antiboby worked very well at dilution 1:1000 in WB using Htr8/SVneo cell line lysates. It gave a specific band at around 100 kDa with very low background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Htr8/SVneo

NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Htr8/SVneo (Cat no:16396-1-AP)

FH

Xiaoping (Verified Customer) (10-14-2021)

Gave good bands around 100 kda in renal cell lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Kidney

FH

Razia Sultana (Verified Customer) (08-20-2020)

The antibody gives good band at around 100KD(100 KD in HK2 cells and 90KD in kidney tissue lysate) along with some non specific bands at around 40-50KD. The trial size gave a band at 130KD which is unusual and lot of non specific bands,but the full size antibody is good. We are satisfied with the purchase

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 in 5% milk
• Cell Tissue Type: Rat and mouse kidney tissue lysate and HK2 cell lysate

FH

JIM (Verified Customer) (03-26-2019)

Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.

• Applications: Western Blot,

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