VDAC1/Porin Monoklonaler Antikörper

VDAC1/Porin Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

23

CloneNo.

1E2C7

Kat-Nr. : 66345-1-Ig

Synonyme

hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC1/Porin

---

• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis using 66345-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  IHC staining of human heart using 66345-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 66345-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66345-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66345-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

"VDAC1/Porin Antibodies" Comparison

View side-by-side comparison of VDAC1/Porin antibodies from other vendors to find the one that best suits your research needs.

Compare now

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, RAW264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66345-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA VDAC1/Porin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: voltage-dependent anion channel 1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: NM_003374
• Gene symbol: VDAC1
• Gene ID (NCBI): 7416
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).

Publikationen

Species	Application	Title
	WB	Proc Natl Acad Sci U S A; Ferroptosis as a target for protection against cardiomyopathy.; Authors - Xuexian Fang
human	WB	Free Radic Biol Med; The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.; Authors - Zhiguo Li
mouse		Diabetes; PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.; Authors - Chenrui Li
mouse	WB	Oxid Med Cell Longev; The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury.; Authors - Wei Tang
mouse	WB	Cell Death Discov; Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice.; Authors - Junqiang Yan
human	WB	Int J Mol Sci; PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.; Authors - Yanqing Li

Verwandte Produkte

VDAC1/Porin Polyclonal antibody

55259-1-AP

VDAC1/2 Polyclonal antibody

10866-1-AP

VDAC2 Monoclonal antibody

66388-1-Ig

VDAC2 Polyclonal antibody

55261-1-AP

VDAC3 Polyclonal antibody

55260-1-AP

COXIV Monoclonal antibody

66110-1-Ig

COXIV Polyclonal antibody

11242-1-AP

Cytochrome c Monoclonal antibody

66264-1-Ig

Bcl-XL Monoclonal antibody

66020-1-Ig

TOM20 Polyclonal antibody

11802-1-AP