BAX Polyklonaler Antikörper

BAX Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

45

Kat-Nr. : 23931-1-AP

Synonyme

Apoptosis regulator BAX, BAX

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 23931-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 23931-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 23931-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 23931-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23931-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23931-1-AP (BAX Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 23931-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23931-1-AP (BAX Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

Empfohlene Verdünnung

Anwendung	Verdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 41 publications below
• IHC: See 2 publications below

Produktinformation

23931-1-AP bindet in WB, IHC, ELISA BAX und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAX fusion protein Ag21068
• Vollständiger Name: BCL2-associated X protein
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014175
• Gene symbol: BAX
• Gene ID (NCBI): 581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

Publikationen

Species	Application	Title
mouse	WB	Br J Pharmacol; Astaxanthin attenuates hepatic damage and mitochondrial dysfunction in non-alcoholic fatty liver disease by up-regulating the FGF21/PGC-1α pathway.; Authors - Liwei Wu
human	WB	Oncotarget; By reducing hexokinase 2, resveratrol induces apoptosis in HCC cells addicted to aerobic glycolysis and inhibits tumor growth in mice.; Authors - Weiqi Dai
human	WB	Aging (Albany NY); SRT2183 impairs ovarian cancer by facilitating autophagy.; Authors - Tingting Sun
human	WB	Int J Oncol; NT21MP negatively regulates paclitaxel-resistant cells by targeting miR‑155‑3p and miR‑155-5p via the CXCR4 pathway in breast cancer.; Authors - Yueyue Wang
human	WB	Nanoscale Res Lett; Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells.; Authors - Mingjun Zhang
human	WB	Oncotarget; AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells.; Authors - Yarong Song

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