Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, HepG2 cells, Neuro-2a cells|
|Positive IHC detected in||human prostate hyperplasia tissue, human prostate cancer tissue, mouse liver tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Sample-dependent, check data in validation data gallery|
10435-1-AP targets BAD in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAD fusion protein Ag0688|
|Full Name||BCL2-associated agonist of cell death|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18-25 kDa|
|GenBank accession number||BC001901|
|Gene ID (NCBI)||572|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer
Oxid Med Cell Longev
Cystathionine β-Synthase Regulates the Proliferation, Migration, and Invasion of Thyroid Carcinoma Cells.
Oxid Med Cell Longev
Dihydroartemisinin Modulates Apoptosis and Autophagy in Multiple Myeloma through the P38/MAPK and Wnt/β-Catenin Signaling Pathways.
Int J Nanomedicine
Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.
Cell Death Dis
CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells.
Targeting ERK enhances the cytotoxic effect of the novel PI3K and mTOR dual inhibitor VS-5584 in preclinical models of pancreatic cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray