BAD Polyclonal antibody

BAD Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 10435-1-AP



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, Neuro-2a cells
Positive IHC detected inhuman prostate hyperplasia tissue, human prostate cancer tissue, mouse liver tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10435-1-AP targets BAD in WB,FC,IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAD fusion protein Ag0688
Full Name BCL2-associated agonist of cell death
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18-25 kDa
GenBank Accession NumberBC001901
Gene Symbol BAD
Gene ID (NCBI) 572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.


Product Specific Protocols
WB protocol for BAD antibody 10435-1-APDownload protocol
IHC protocol for BAD antibody 10435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other