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  • KD/KO Validated

human BCL2 Polyclonal antibody

human BCL2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (9)

Applications

WB, IP, IF, IHC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12789-1-AP

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells
Positive IP detected inHL-60 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12789-1-AP targets human BCL2 in WB, IP, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, Goat, chicken, sheep, rabbit, fish, zebrafish, hamster, pig, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human BCL2 fusion protein Ag3508
Full Name B-cell CLL/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
RRIDAB_2227948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

 a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.


Protocols

Product Specific Protocols
WB protocol for human BCL2 antibody 12789-1-APDownload protocol
IHC protocol for human BCL2 antibody 12789-1-APDownload protocol
IP protocol for human BCL2 antibody 12789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
WB,CoIP

Cell Metab

Exercise-activated hepatic autophagy via the FN1-α5β1 integrin pathway drives metabolic benefits of exercise

Authors - Kenta Kuramoto
pigWB

Cell Stem Cell

Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation

Authors - Jiaowei Wang
humanWB

ACS Nano

Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer.

Authors - Danielle M Valcourt
WB

ACS Nano

Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy

Authors - Qiqi Zhang

ACS Nano

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Authors - Xirui Xin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandru (Verified Customer) (11-21-2023)

The antibody works well in recognising Bcl-2 in western blots. Great product!

  • Applications: Western Blot
  • Cell Tissue Type: HUVEC
FH

Jia (Verified Customer) (03-01-2022)

The antibody works well for Bcl2, we recommend it.

  • Applications: Western Blot
FH

Giyeong (Verified Customer) (02-18-2022)

Works well with sharp distinct vand

  • Applications: Western Blot
FH

Morgan (Verified Customer) (10-26-2021)

This product worked really well. I attached photos to show how clean the blot came out when used on ovarian cancer cells (OV90s). I used 1:500 for the first use, but I could definitely use less antibody next time.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: OV90 (Ovarian cancer cell line)
human BCL2 Antibody Western Blot validation (1:500 dilution) in OV90 (Ovarian cancer cell line) (Cat no:12789-1-AP)
FH

Tom (Verified Customer) (10-09-2020)

This antibody recognizes Bcl-2 at the correct molecular weight. Highly recommended!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,05 µl/ million cells with Bcl2 antibody (12789-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody only and in red primary (12789-1-AP) +secondary antibody.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0,05µl/10^6 cells
  • Cell Tissue Type: B cells
human BCL2 Antibody Flow Cytometry validation (0,05µl/10^6 cells dilution) in B cells (Cat no:12789-1-AP)