BID Polyclonal antibody

• Featured Product
• KD/KO Validated

BID Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

60

Cat no : 10988-1-AP

Synonyms

BID, FP497, p11 BID, p22 BID

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Validation Data Gallery

• 

  WB analysis using 10988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10988-1-AP

  WB result of BID antibody (10988-1-AP, 1:8000) with si-Control and si-BID transfected HeLa cells.

• 

  WB analysis of HeLa using 10988-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10988-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10988-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10988-1-AP (BID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10988-1-AP

  IP result of anti-BID (IP:10988-1-AP, 4ug; Detection:10988-1-AP 1:500) with HeLa cells lysate 1280 ug.

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Tested Applications

• Positive WB detected in: A431 cells, human brain tissue, Tunicamycin treated HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 57 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10988-1-AP targets BID in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BID fusion protein Ag1445
• Full Name: BH3 interacting domain death agonist
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC009197
• Gene Symbol: BID
• Gene ID (NCBI): 637
• RRID: AB_2065624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)

Publications

Species	Application	Title
rat	WB	Bioact Mater; A ROS-responsive, self-immolative and self-reporting hydrogen sulfide donor with multiple biological activities for the treatment of myocardial infarction.; Authors - Mengyun Yao
mouse	WB	Environ Pollut; Silica nanoparticles inducing the apoptosis via microRNA-450b-3p targeting MTCH2 in mice and spermatocyte cell.; Authors - Guiqing Zhou
rat	WB	Cell Mol Life Sci; Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B.; Authors - Katia Fettucciari
human	WB	Int J Cancer; Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.; Authors - Liu Chaoyang C
human	WB	Free Radic Biol Med; Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma.; Authors - Gong Ke K
rat	WB	Stem Cell Res Ther; Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.; Authors - Chaoling Yao

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