ATG12 Polyklonaler Antikörper

ATG12 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Rezensionen (1)

Kat-Nr. : 11122-1-AP

Synonyme

APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12

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Galerie der Validierungsdaten

• 

  WB analysis of human bladder using 11122-1-AP

  human bladder tissue were subjected to SDS PAGE followed by western blot with 11122-1-AP (ATG12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated HEK-293 using 11122-1-AP

  UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 11122-1-AP (ATG12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11122-1-AP

  IP result of anti-ATG12 (IP:11122-1-AP, 4ug; Detection:11122-1-AP 1:300) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human prostate cancer using 11122-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11122-1-AP (ATG12 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blasengewebe, UV treated HEK-293
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11122-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA ATG12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG12 fusion protein Ag1586
• Vollständiger Name: ATG12 autophagy related 12 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC011033
• Gene symbol: ATG12
• Gene ID (NCBI): 9140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

Publikationen

Species	Application	Title
	WB	Mol Cell; AMPK-Dependent Phosphorylation of GAPDH Triggers Sirt1 Activation and Is Necessary for Autophagy upon Glucose Starvation.; Authors - Chunmei Chang
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	IHC	Theranostics; Down-regulated miR-23a Contributes to the Metastasis of Cutaneous Melanoma by Promoting Autophagy.; Authors - Weinan Guo
human	WB	Cancer Lett; Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy.; Authors - Bo You
human	WB	Cell Death Dis; The lncRNA HULC functions as an oncogene by targeting ATG7 and ITGB1 in epithelial ovarian carcinoma.; Authors - Shuo Chen
human	WB	Biochem Pharmacol; Ginsenoside Rg5 induces G2/M phase arrest, apoptosis and autophagy via regulating ROS-mediated MAPK pathways against human gastric cancer.; Authors - Yannan Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

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