ATG3 Polyclonal antibody

ATG3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 11262-2-AP

Synonyms

APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, hApg3, PC3 96, Protein PC3 96

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Validation Data Gallery

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  WB analysis of mouse kidney using 11262-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11262-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11262-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11262-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 11262-2-AP (ATG3 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 11262-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 11262-2-AP (ATG3 Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 11262-2-AP

  Immunofluorescent analysis of HEK-293 cells using 11262-2-AP (ATG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse kidney tissue, K-562 cells, PC-12 cells, THP-1 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11262-2-AP targets ATG3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG3 fusion protein Ag1785
• Full Name: ATG3 autophagy related 3 homolog (S. cerevisiae)
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 36-40 kDa
• GenBank accession number: BC024221
• Gene symbol: ATG3
• Gene ID (NCBI): 64422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.; Authors - Bo Wu
pig	WB	Chemosphere; Genome-wide transcriptional profiling and functional analysis reveal miR-330-MAPK15 axis involving in cellular responses to deoxynivalenol exposure.; Authors - Haifei Wang
mouse	WB	Front Cell Dev Biol; Aquaporin-8 transports hydrogen peroxide to regulate granulosa cell autophagy; Authors - Binbin Huang
mouse	WB	Front Immunol; Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages.; Authors - Minmin Wu
human	WB	Oncotarget; Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.; Authors - Yin-Ling Xiu
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong