LC3A/B Monoclonal antibody

LC3A/B Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B2B5

Cat no : 66139-1-Ig

Synonyms

LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66139-1-Ig is LC3A/B Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, mouse
Cited Reactivityhuman, mouse, rat, hamster, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name microtubule-associated protein 1 light chain 3 alpha
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa, 16 kDa
GenBank accession numberNM_032514
Gene symbol MAP1LC3A
Gene ID (NCBI) 84557
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.

Publications

SpeciesApplicationTitle
mouseIF

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
mouse,ratWB,IP

Cell Death Dis

Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy.

Authors - Jichao Wu
pigIF

Cell Prolif

S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos.

Authors - Ying-Jie Niu
pigWB

Aging (Albany NY)

Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.

Authors - Ying-Jie Niu
hamsterIF

Front Mol Neurosci

Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.

Authors - Zhiqi Song
mouseWB,IF

Stem Cell Res Ther

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Authors - Caixia Jin