|Sample-dependent, check data in validation data gallery|
The immunogen of 66139-1-Ig is LC3A/B Fusion Protein expressed in E. coli.
|Tested Reactivity||human, pig, mouse|
|Cited Reactivity||human, mouse, rat, hamster, pig|
|Host / Isotype||Mouse / IgG1|
|Full Name||microtubule-associated protein 1 light chain 3 alpha|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14 kDa, 16 kDa|
|GenBank accession number||NM_032514|
|Gene ID (NCBI)||84557|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.
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