ATG7 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATG7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

135

Reviews (4)

Cat no : 10088-2-AP

Synonyms

GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L

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Validation Data Gallery

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  WB analysis using 10088-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10088-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-1080 using 10088-2-AP

  IP result of anti-ATG7 (IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with HT-1080 cells lysate 3600 ug.

• 

  IP experiment of NIH/3T3 using 10088-2-AP

  IP result of anti-ATG7 (IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with NIH/3T3 cells lysate 1600 ug.

• 

  IHC staining of human cervical cancer using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10088-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10088-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (10088-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, THP-1 cells, U2OS cells
• Positive IP detected in: HT-1080 cells, NIH/3T3 cells
• Positive IHC detected in: human cervical cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 21 publications below
• WB: See 122 publications below
• IHC: See 16 publications below
• IF: See 12 publications below
• CoIP: See 1 publications below

Product Information

10088-2-AP targets ATG7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken, hamster, goat, turtle, prawn
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG7 fusion protein Ag0142
• Full Name: ATG7 autophagy related 7 homolog (S. cerevisiae)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 68-78 kDa
• GenBank Accession Number: BC000091
• Gene Symbol: ATG7
• Gene ID (NCBI): 10533
• RRID: AB_2062351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human	WB	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang ; KD Validated
human	WB	Cell Death Differ; STING directly activates autophagy to tune the innate immune response.; Authors - Dong Liu
mouse	WB, IHC	Autophagy; Autophagy regulates functional differentiation of mammary epithelial cells.; Authors - Jessica Elswood
rat	WB	Autophagy; Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis.; Authors - Sheng Zhang
human,mouse	WB	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (10-03-2024)

Used this antibody for colon carcinoma cell line and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (08-09-2023)

Used for Caco2 cells and mice colon tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Kamal (Verified Customer) (06-29-2023)

Atg7 band showed up better than from other companies.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue

ATG7 Antibody Western Blot validation (1:2000 dilution) in Mouse Liver tissue (Cat no:10088-2-AP)

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

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