LC3B-Specific Polyclonal antibody

LC3B-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

194

Reviews (2)

Cat no : 18725-1-AP

Synonyms

LC3, LC3B, LC3B-Specific, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B

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Validation Data Gallery

• 

  WB analysis of human brain using 18725-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TN treated Hela using 18725-1-AP

  TN treated Hela cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 18725-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18725-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18725-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated HEK-293 using 18725-1-AP

  UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18725-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18725-1-AP

  Immunofluorescent analysis of -20℃ Ethanol fixed CQ treated HepG2 cells (left) and untreated HepG2 cells (right) using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin).

• 

  IF Staining of HepG2 using 18725-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed CQ treated HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 18725-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18725-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using LC3B-Specific antibody (18725-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 18725-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 18725-1-AP

  Immunofluorescent analysis of starvation treated HEK-293 cells using 18725-1-AP (LC3B-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with ethanol at -20 °C. 20 mM chloroquine was used to block the autophagy flux.

• 

  FC experiment of HeLa using 18725-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LC3B-Specific (18725-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human brain tissue, MCF-7 cells, TN treated Hela, A549 cells, UV treated HEK-293, mouse brain tissue, HepG2 cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, Chloroquine treated HeLa cells, Starvation treated HepG2 cells
• Positive FC detected in: HeLa cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 151 publications below
• IHC: See 25 publications below
• IF: See 53 publications below
• IP: See 1 publications below

Product Information

18725-1-AP targets LC3B-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: microtubule-associated protein 1 light chain 3 beta
• Calculated molecular weight: 15 kDa
• Observed molecular weight: 15 kDa, 18 kDa
• GenBank accession number: NM_022818
• Gene symbol: MAP1LC3B
• Gene ID (NCBI): 81631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.

Publications

Species	Application	Title
mouse	IF	Exp Cell Res; Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice.; Authors - Yajie Liu
human	WB	J Cell Physiol; Nix/BNIP3L-dependent mitophagy accounts for airway epithelial cell injury induced by cigarette smoke.; Authors - Ming Zhang
human,mouse	WB,IHC	Cancer Cell Int; Gambogic acid induces autophagy and combines synergistically with chloroquine to suppress pancreatic cancer by increasing the accumulation of reactive oxygen species.; Authors - Hongcheng Wang
mouse	WB	J Mol Histol; Autophagy promotes hepatic differentiation of hepatic progenitor cells by regulating the Wnt/β-catenin signaling pathway.; Authors - Zhenzeng Ma
human	WB,IF	Toxins (Basel); Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells.; Authors - Jing Wu
mouse	WB	J Immunol Res; Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice.; Authors - Meng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:300. Immunohistochemistry labelling of primary rat cortical neurons using LC3B-Specific Polyclonal antibody at dilution of 1:500 showed strong labelling.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:300 (WB), 1:500 (IHC)
• Cell Tissue Type: NSC34

FH

Tanusree (Verified Customer) (12-03-2019)

The antibody works great in Western blotting analysis.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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