LC3B-Specific Polyclonal antibody
LC3B-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
194
Cat no : 18725-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue, MCF-7 cells, TN treated Hela, A549 cells, UV treated HEK-293, mouse brain tissue, HepG2 cells |
Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, Chloroquine treated HeLa cells, Starvation treated HepG2 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 151 publications below |
IHC | See 25 publications below |
IF | See 53 publications below |
IP | See 1 publications below |
Product Information
18725-1-AP targets LC3B-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | microtubule-associated protein 1 light chain 3 beta |
Calculated molecular weight | 15 kDa |
Observed molecular weight | 15 kDa, 18 kDa |
GenBank accession number | NM_022818 |
Gene symbol | MAP1LC3B |
Gene ID (NCBI) | 81631 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
IHC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
IF protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Cell Res Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice. | ||
J Cell Physiol Nix/BNIP3L-dependent mitophagy accounts for airway epithelial cell injury induced by cigarette smoke. | ||
Cancer Cell Int Gambogic acid induces autophagy and combines synergistically with chloroquine to suppress pancreatic cancer by increasing the accumulation of reactive oxygen species. | ||
J Mol Histol Autophagy promotes hepatic differentiation of hepatic progenitor cells by regulating the Wnt/β-catenin signaling pathway. | ||
Toxins (Basel) Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells. | ||
J Immunol Res Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:300. Immunohistochemistry labelling of primary rat cortical neurons using LC3B-Specific Polyclonal antibody at dilution of 1:500 showed strong labelling.
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FH Tanusree (Verified Customer) (12-03-2019) | The antibody works great in Western blotting analysis.
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