Validation Data Gallery
|Positive WB detected in||human brain tissue, MCF-7 cells, TN treated Hela, A549 cells, UV treated HEK-293, mouse brain tissue, HepG2 cells|
|Positive IHC detected in||mouse brain tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, Chloroquine treated HeLa cells, Starvation treated HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18725-1-AP targets LC3B-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Full Name||microtubule-associated protein 1 light chain 3 beta|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15 kDa, 18 kDa|
|GenBank accession number||NM_022818|
|Gene ID (NCBI)||81631|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.
Exp Cell Res
Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice.
J Cell Physiol
Nix/BNIP3L-dependent mitophagy accounts for airway epithelial cell injury induced by cigarette smoke.
Cancer Cell Int
Gambogic acid induces autophagy and combines synergistically with chloroquine to suppress pancreatic cancer by increasing the accumulation of reactive oxygen species.
J Mol Histol
Autophagy promotes hepatic differentiation of hepatic progenitor cells by regulating the Wnt/β-catenin signaling pathway.
Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells.
J Immunol Res
Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:300. Immunohistochemistry labelling of primary rat cortical neurons using LC3B-Specific Polyclonal antibody at dilution of 1:500 showed strong labelling.
Tanusree (Verified Customer) (12-03-2019)
The antibody works great in Western blotting analysis.