Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, mouse kidney tissue, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10181-2-AP targets ATG5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, monkey, pig, bovine, grouper|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG5 fusion protein Ag0214|
|Full Name||ATG5 autophagy related 5 homolog (S. cerevisiae)|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32 kDa, 40-45 kDa, 50-55 kDa|
|GenBank accession number||BC002699|
|Gene ID (NCBI)||9474|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).
Signal Transduct Target Ther
Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy
Degradation of lipid droplets by chimeric autophagy-tethering compounds.
Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.
Adv Sci (Weinh)
SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle
Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models
J Clin Invest
Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Priya (Verified Customer) (08-09-2023)
Used for Caco2 cells and mice colon tissue
Kamal (Verified Customer) (06-29-2023)
Works well with TBST. Atg5-12 complex (56 kDa) and Atg5 (33kDa) bands came out nice and clear.
Benjamin (Verified Customer) (01-07-2020)
Works well in western blot in 3% milk TBS-T