ATG5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATG5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

314

Reviews (5)

Cat no : 10181-2-AP

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

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Validation Data Gallery

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  WB analysis using 10181-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10181-2-AP

  WB result of ATG5 antibody (10181-2-AP; 1:2000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells.

• 

  WB analysis using 10181-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10181-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10181-2-AP

  IP result of anti-ATG5 (IP:10181-2-AP, 4ug; Detection:10181-2-AP 1:3000) with HeLa cells lysate 1760 ug.

• 

  IHC staining of human colon cancer using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10181-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse kidney tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 43 publications below
• WB: See 288 publications below
• IHC: See 27 publications below
• IF: See 25 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Product Information

10181-2-AP targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine, hamster, goat, grouper
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG5 fusion protein Ag0214
• Full Name: ATG5 autophagy related 5 homolog (S. cerevisiae)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa, 40-45 kDa, 50-55 kDa
• GenBank Accession Number: BC002699
• Gene Symbol: ATG5
• Gene ID (NCBI): 9474
• RRID: AB_2062045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
human	WB	Cell Res; Degradation of lipid droplets by chimeric autophagy-tethering compounds.; Authors - Yuhua Fu ; KO Validated
human	WB	Mol Cell; Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.; Authors - Lu Yubing Y ; KD Validated
human	WB	Adv Sci (Weinh); SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle; Authors - Xiaowei She
human		Autophagy; Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models; Authors - Panagiota Mavroeidi
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (08-09-2023)

Used for Caco2 cells and mice colon tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Kamal (Verified Customer) (06-29-2023)

Works well with TBST. Atg5-12 complex (56 kDa) and Atg5 (33kDa) bands came out nice and clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Liver tissue

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Benjamin (Verified Customer) (01-07-2020)

Works well in western blot in 3% milk TBS-T

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK239 Mammalian Cell Lysate

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