|Positive WB detected in||mouse spleen tissue, MCF-7 cells, Jurkat cells, HEK-293T cells|
|Positive IP detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19812-1-AP is ATG16L1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG16L1 fusion protein Ag13844|
|Full Name||ATG16 autophagy related 16-like 1 (S. cerevisiae)|
|Calculated molecular weight||607 aa, 68 kDa|
|Observed molecular weight||63-71 kDa|
|GenBank accession number||BC000061|
|Gene ID (NCBI)||55054|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
X (Verified Customer) (01-18-2021)
It is OK to use it in WB with human postmortem brain lysate. There is band with MW as expected, although there is other unidentified bands.