ATG16L1 Polyclonal antibody

ATG16L1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (5)

Cat no : 19812-1-AP

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, IBD10, WDR30

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Validation Data Gallery

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  WB analysis of mouse spleen using 19812-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 19812-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19812-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19812-1-AP (ATG16L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 19812-1-AP

  IP result of anti-ATG16L1 (IP:19812-1-AP, 4ug; Detection:19812-1-AP 1:300) with MCF-7 cells lysate 2000ug.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, MCF-7 cells, Jurkat cells, HEK-293T cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 19812-1-AP is ATG16L1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG16L1 fusion protein Ag13844
• Full Name: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 607 aa, 68 kDa
• Observed Molecular Weight: 63-71 kDa
• GenBank Accession Number: BC000061
• Gene Symbol: ATG16L1
• Gene ID (NCBI): 55054
• RRID: AB_10695631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Publications

Species	Application	Title
human	IF	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie
human	IF	Nat Commun; Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.; Authors - Shuang-Zhou Peng
human	WB	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human	WB,IHC	Cancer Lett; Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy.; Authors - Bo You
human	WB	Clin Transl Immunology; Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA-874-3p.; Authors - Hou-Long Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (07-31-2023)

Used for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 2000
• Cell Tissue Type: Caco2, Mice colon

FH

X (Verified Customer) (01-18-2021)

It is OK to use it in WB with human postmortem brain lysate. There is band with MW as expected, although there is other unidentified bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human postmortem brain lysate

ATG16L1 Antibody Western Blot validation (1:1000 dilution) in human postmortem brain lysate (Cat no:19812-1-AP)