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  • KD/KO Validated

ATG5 Monoclonal antibody

ATG5 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgM


human, mouse







Cat no : 60061-1-Ig


APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60061-1-Ig targets ATG5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen ATG5 fusion protein Ag0214
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC002699
Gene Symbol ATG5
Gene ID (NCBI) 9474
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.


Product Specific Protocols
WB protocol for ATG5 antibody 60061-1-IgDownload protocol
IHC protocol for ATG5 antibody 60061-1-IgDownload protocol
IF protocol for ATG5 antibody 60061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.

Authors - Qian Huang


The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Authors - Andreas Struchtrup

Cancer Cell Int

PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth

Authors - Xiangyan Zhang

Cell Biosci

A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.

Authors - Juan Yin

Mol Med Rep

Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.

Authors - Shuo Wang
  • KD Validated

Cells Dev

Enhanced autophagy suppresses inflammation-mediated bone loss through ROCK1 signaling in bone marrow mesenchymal stem cells.

Authors - Jingjing Zheng