Flow Cytometry Applications

Learn about the various applications the flow cytometry is used for.

Flow cytometry applications

• Analysis of plasma membrane receptor expression

• Analysis of cell viability, apoptosis, and necrosis

• Cell phenotyping

• Analysis of cell cycle

• Cell sorting

• Analysis of intracellular protein expression

• Biomarker detection

• Cell counting

• Quantification and isolation of Circulation Tumor Cells (CTCs)

Table of cell-line-specific markers to identify or isolate CTCs by Flow cytometry:

Cell line	Cancer Type	Flow Cytometry marker
A549	NSCLC (Adenocarcinoma)	ALDH, CD133, CD90, CD44+, HPRT
NCI – H226	NSCLC (SCC)	EpCAM, CK8
NCI – H460	NSCLC (LCC)	PD-L1, CD318, EpCAM, Cytokeratin, HPRT
NCI – H69	SCLC	CD133, E-Cadherin
MCF7	BC Luminal A (ER+/PR+)	CD166,  Cytokeratin, CK18, CK19, E-Cadherin, CD24, EpCAM
T47D	BC Luminal A (ER+/PR+)	Cytokeratin, E-Cadherin, CK19, CD24, EpCAM,
SKBR3	BC ER-/PR- HER2+	CK19, HER2, CD164, CD109, ALDH1A3, ALDH3B2, EpCAM, CD24
AU565	BC ER-/PR- HER2+	HER2
MDA-MB-231	TNBC/Claudin Low	CD166, CD318, CD133, [CD44+/CD24-]
MDA-MB-468	TNBC	CD44, CD133
4T1	Mouse TNBC	EpCaM
Hep3B	HCC	CD13, CD133, EpCAM, CD44-, CD24+
HepG2	Hepatoblastoma	CD133, EpCAM, CD44
HuH6	Hepatoblastoma	CD133
PC3	Prostate	EpCAM, ZO-1, CD44, CK8/18/19
DU145	Prostate	CD44
LNCaP	Prostate	EpCAM, CK8/18/19
ES-2	Ovarian	EpCAM, E-Cadherin-, N-Cadherin+
PEO-1	Ovarian	EpCAM, E-Cadherin+, N-Cadherin-
OVCAR-3	Ovarian	EpCAM, N-Cadherin-, CD44
Colorectal Primary CTCs	Colorectal	CK19, MUC1, CD44, CD133, ALDH1, CD47
HCT116	Colorectal	CK19, EpCAM, CD133
HT-29	Colorectal	EpCAM, CK20, CD26, CD44, E-Cadherin
T84	Colorectal	CD26, EpCAM, E-Cadherin
SKMel24	Melanoma	CD200
SkMel28	Melanoma	S100b, GP100, MCAM, MCSP/GPX4, Melan-A
WM2664	Melanoma	CD200, MUC18/CD146, S100
AML t(8:21) (patients)	Leukemia	CD34, HLA-DR, CD13, CD36^low, CD105^low, CD71^low
AML t(15:17) (patients)	Leukemia	CD34^low, HLA-DR^low, CD13, CD36^low, CD105^low, CD71^low
AML inv (16) (patients)	Leukemia	CD34, HLA-DR, CD13, CD36^low, CD105^low, CD71^low
AML cell lines (U937, KG1a, MV4-11)	AML	CD44, CD34, CD33
(OSU-CLL)	CLL Chronic Lymphocytic Leukemia – immortalised patient sample	CD19, CD5, CD20, CD43, CD80, CD86, kappa/lambda ratio
Non Hodgkin’s Lymphoma	Lymphoma	Kappa/lambda, BCL-2, LCA, CD30, CD43, CD20, PAX-5
Hodgkin’s Lymphoma	Lymphoma	CD15, CD30, CD57
Burkitt’s Lymphoma	Lymphoma	CD10

Abbreviations:

NSCLC – non small cell lung carcinoma

SCC – squamous cell carcinoma

LCC – large cell carcinoma

SCLC – small cell lung carcinoma

BC – breast cancer

TNBC – triple negative breast cancer

HCC – hepatocellular carcinoma

AML – acute myeloid leukemia

CLL – chronic lymphocytic leukemia