CDCP1 Polyclonal antibody

CDCP1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 12754-1-AP

Synonyms

CD318, CDCP1, Membrane glycoprotein gp140, SIMA135, TRASK



Tested Applications

Positive WB detected inA549 cells, LNCaP cells, PC-3 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12754-1-AP targets CDCP1 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDCP1 fusion protein Ag3439
Full Name CUB domain containing protein 1
Calculated molecular weight 836 aa, 93 kDa
Observed molecular weight 72 kDa, 130 kDa
GenBank accession numberBC021099
Gene symbol CDCP1
Gene ID (NCBI) 64866
RRIDAB_2078799
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCP1, also named as CD318, TRASK and SIMA 135, is a 135-140 kDa transmembrane protein that contains three CUB domains within the extracellular region and a hexalysine stretch within the cytoplasmic region. A soluble form with MW 80 kDa (p80) is cleaved at an unidentified site on the N-terminal side. CDCP1 is a novel marker for hematopoietic stem cells.

Protocols

Product Specific Protocols
WB protocol for CDCP1 antibody 12754-1-APDownload protocol
FC protocol for CDCP1 antibody 12754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity.

Authors - Wanzun Lin
humanWB

Aging (Albany NY)

The emerging role of the MiR-1272-ADAM9-CDCP1 signaling pathway in the progression of glioma.

Authors - Fei Geng