CD57 Monoclonal antibody

CD57 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4A11

Cat no : 66711-1-Ig

Synonyms

B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1



Tested Applications

Positive WB detected inK-562 cells, mouse brain tissue, Jurkat cells, PC-3 cells, SH-SY5Y cells, U-251 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66711-1-Ig targets CD57 in WB, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD57 fusion protein Ag13593
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 100 kDa
GenBank accession numberBC010466
Gene symbol B3GAT1
Gene ID (NCBI) 27087
RRIDAB_2882062
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

Protocols

Product Specific Protocols
WB protocol for CD57 antibody 66711-1-IgDownload protocol
FC protocol for CD57 antibody 66711-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols