• Featured Product
  • KD/KO Validated

HPRT1 Polyclonal antibody

HPRT1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15059-1-AP

Synonyms

HGPRT, HGPRTase, HPRT, HPRT1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, mouse liver tissue, MCF-7 cells, HepG2 cells, Jurkat cells, Y79 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15059-1-AP targets HPRT1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPRT1 fusion protein Ag7044
Full Name hypoxanthine phosphoribosyltransferase 1
Calculated molecular weight 25 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC000578
Gene symbol HPRT1
Gene ID (NCBI) 3251
RRIDAB_10638622
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPRT1, also named HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT1 is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout.

Protocols

Product Specific Protocols
WB protocol for HPRT1 antibody 15059-1-APDownload protocol
IHC protocol for HPRT1 antibody 15059-1-APDownload protocol
IF protocol for HPRT1 antibody 15059-1-APDownload protocol
IP protocol for HPRT1 antibody 15059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.

Authors - Xiaoyan Yang
ratWB

J Clin Invest

ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peri-partum cardiomyopathy in mice.

Authors - Hidemichi Kouzu
mouseWB

Cell Rep

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways

Authors - Tatsuki Yasuda
human,mouseWB

Br J Cancer

Blocking of oestrogen signals improves anti-tumour effect regardless of oestrogen receptor alpha expression in cancer cells

Authors - Nabeel Kajihara
humanWB

EMBO Rep

MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.

Authors - Gongwei Wu
mouseWB

Elife

Aging is associated with increased brain iron through cortex-derived hepcidin expression.

Authors - Tatsuya Sato