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BSAP,PAX5 Polyclonal antibody

BSAP,PAX5 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 26709-1-AP

Synonyms

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5



Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IP detected inRamos cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 3 publications below

Product Information

26709-1-AP targets BSAP,PAX5 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BSAP,PAX5 fusion protein Ag25147
Full Name paired box 5
Calculated molecular weight 391 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC156927
Gene symbol PAX5
Gene ID (NCBI) 5079
RRIDAB_2880608
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Protocols

Product Specific Protocols
WB protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
IHC protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
IP protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
FC protocol for BSAP,PAX5 antibody 26709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.

Authors - Runzhi Huang
humanIHC

J Cutan Pathol

Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.

Authors - Jennifer S Ko
mouseIHC

Mol Oncol

miR-126 downregulates CXCL12 expression in intestinal epithelial cells to suppress the recruitment and function of macrophages and tumorigenesis in a murine model of colitis-associated colorectal cancer

Authors - Shuai Wu