DPP4/CD26 Polyclonal antibody

DPP4/CD26 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 29403-1-AP

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103



Tested Applications

Positive WB detected inCaco-2 cells, HT-29 cells
Positive FC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

29403-1-AP targets DPP4/CD26 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPP4/CD26 fusion protein Ag30160
Full Name dipeptidyl-peptidase 4
Calculated molecular weight 88 kDa
Observed molecular weight110 kDa
GenBank accession numberBC013329
Gene symbol DPP4
Gene ID (NCBI) 1803
RRIDAB_2918296
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580). In addition to the 110 kDa monomeric form, a 290 kDa active dimeric form and a 60 kDa fragment of CD26 have also been reported (PMID: 9654125; 7905271).

Protocols

Product Specific Protocols
WB protocol for DPP4/CD26 antibody 29403-1-APDownload protocol
FC protocol for DPP4/CD26 antibody 29403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Allergy

High-fat diet and palmitic acid amplify airway type 2 inflammation

Authors - Kris Genelyn Dimasuay
mouseWB

Biomed Pharmacother

Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa

Authors - Yijing Yang
humanWB

Anal Chem

Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells

Authors - Qiuyu Gong