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  • KD/KO Validated

MUC1/CA15-3 C-terminal Polyclonal antibody

MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 23614-1-AP

Synonyms

CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, MUC1/CA15-3 C-terminal, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin



Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue, human tonsillitis tissue, human breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

23614-1-AP targets MUC1/CA15-3 C-terminal in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC1/CA15-3 C-terminal fusion protein Ag20366
Full Name mucin 1, cell surface associated
Calculated molecular weight 1264 aa, 123 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC120975
Gene symbol MUC1
Gene ID (NCBI) 4582
RRIDAB_2879304
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

Protocols

Product Specific Protocols
WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
FC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

ACS Sens

Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.

Authors - Xiaoyi Gao
humanWB

Cell Cycle

LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.

Authors - Yue Li
humanWB

Pathol Res Pract

MUC1 promotes cancer stemness and predicts poor prognosis in osteosarcoma

Authors - Jian Liu
  • KD Validated
humanWB,IHC

Cell Death Discov

Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling.

Authors - Zongwen Liang
mouseWB

Cell Death Discov

Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.

Authors - Wenwen Wang
humanWB,IHC

Front Oncol

A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma

Authors - Xinxing Wang