- Featured Product
- KD/KO Validated
MUC1/CA15-3 C-terminal Polyclonal antibody
MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA
Cat no : 23614-1-AP
Validation Data Gallery
|Positive WB detected in
|DU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells
|Positive IHC detected in
|human pancreas cancer tissue, human tonsillitis tissue, human breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
23614-1-AP targets MUC1/CA15-3 C-terminal in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|MUC1/CA15-3 C-terminal fusion protein Ag20366
|mucin 1, cell surface associated
|Calculated molecular weight
|1264 aa, 123 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.
|Product Specific Protocols
|WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP
|IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP
|FC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP
|Click here to view our Standard Protocols
Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.
LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.
Pathol Res Pract
MUC1 promotes cancer stemness and predicts poor prognosis in osteosarcoma
Cell Death Discov
Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling.
Cell Death Discov
Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.
A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma