Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human M-CSF (Cat no: HZ-1192) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.
 

Proteintech M-CSF (Cat no: HZ-1192) demonstrates equivalent fold induction and a 2-fold better EC50 compared to leading competitors. Recombinant human M-CSF stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.

Purity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human M-CSF ( Cat no: HZ-1192-GMP) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.7-4.0 ng/mL.

Recombinant human M-CSF(Cat.NO. HZ-1192) stimulates proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human M-CSF (5 ng/mL) is neutralized by mouse anti-human M-CSF monoclonal antibody 69033-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.4 μg/mL. The control mouse anti-human M-CSF monoclonal antibody 69533-1-Ig could recognize human M-CSF, but could not neutralize human M-CSF at serial does at the range of 0.976563-16000ng/mL(green curve, refer to top X-right Y).

Indirect ELISA was carried out by coating recombinant Human M-CSF (Cat.NO. HZ-1192) at 70 ng/well followed by blocking and adding serial diluted M-CSF antibody 69033-1-Ig and 69533-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.