CoraLite® Plus 750 Anti-Human M-CSF (3G12E6)

M-CSF Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

3G12E6

Cat no : CL750-65283

Synonyms

M-CSF, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Proteoglycan macrophage colony-stimulating factor, 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim, M CSF, Macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, MCSF

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Validation Data Gallery

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  FC experiment of human PBMCs using CL750-65283

  1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human M-CSF (CL750-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65283

  1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human M-CSF (CL750-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human M-CSF (CL750-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Tested Applications

• Positive FC detected in: LPS and Brefeldin A treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65283 targets M-CSF in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: M-CSF fusion protein HZ-1192
• Full Name: colony stimulating factor 1 (macrophage)
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: BC021117
• Gene Symbol: M-CSF
• Gene ID (NCBI): 1435
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)